en ميکروب شناسي Microbiology تحقيقي Original Paper <strong>Background and objectives:</strong> Bacterial contamination of wounds is a serious problem, particularly in burn patients. Gram-positive bacteria are the predominant cause of infection in newly hospitalized burn cases. This study aimed to survey the prevalence and antibiotic resistance pattern of gram-positive bacterial isolates among burn patients in Rasht, North of Iran.<br> <strong>Methods:</strong> This cross-sectional study was conducted on burn patients with a positive culture for gram-positive isolates who were hospitalized in the Velayat Burn Center in Rasht, North of Iran, during 2017-2020. The isolates were identified using standard microbiological methods. Moreover, the antibiotic resistance pattern was determined by the disk diffusion method.<br> <strong>Results:</strong> During the study period, 671 bacterial cultures were obtained, of which a total of 16 gram-positive isolates were taken from the patients. The frequency of coagulase-negative staphylococci (CoNS), <em>Staphylococcus aureus, </em>and <em>Enterococcus </em>spp. was 68.7%, 18.8%, and 12.5%, respectively. In addition, the highest rate of resistance in CoNS isolates was against trimethoprim/sulfamethoxazole. The highest rate of resistant among <em>S. aureus</em> isolates was recorded against penicillin. Moreover, <em>Enterococcus faecalis</em> isolates showed a high level of resistance to ampicillin, erythromycin, tetracycline, gentamicin, and ciprofloxacin. All isolates were susceptible to teicoplanin. Moreover, the frequency of methicillin-resistant <em>S. aureus </em>isolates was 66.7%.<br> <strong>Conclusion:</strong> Given the increasing prevalence of drug-resistant strains, especially in susceptible burn patients, it is imperative to analyze the bacterial etiology of nosocomial infections periodically and epidemiologically. Staphylococcus aureus,Enterococcus,Burns,Gram-positive bacterial infections, 52 57 http://mlj.goums.ac.ir/browse.php?a_code=A-10-1088-1&slc_lang=en&sid=1 Mobina Hosseini vahid.s13@gmail.com 100319475328460020982 100319475328460020982 No Razi Clinical Research Development Unit, Razi Hospital, Guilan University of Medical Sciences, Rasht, Iran Meysam Hasannejad-Bibalan meysam_hasannejad@yahoo.com 100319475328460020983 100319475328460020983 No Department of Microbiology, School of Medicine, Guilan University of Medical Sciences, Rasht, Iran Tofigh Yaghoubi tofigh.yaghubik@gmail.com 100319475328460020984 100319475328460020984 No Razi Clinical Research Development Unit, Razi Hospital, Guilan University of Medical Sciences, Rasht, Iran Mohammadreza Mobayen kaveh.mohammad99@gmail.com 100319475328460020985 100319475328460020985 No Burn and Regenerative Medicine Research Center, Guilan University of Medical Sciences, Rasht, Iran Parisa Khoshdoz parisakhm@yahoo.com 100319475328460020986 100319475328460020986 No Razi Clinical Research Development Unit, Razi Hospital, Guilan University of Medical Sciences, Rasht, Iran Sara Khoshdoz sarakhm73@yahoo.com 100319475328460020987 100319475328460020987 No Razi Clinical Research Development Unit, Razi Hospital, Guilan University of Medical Sciences, Rasht, Iran Niloofar Faraji niloofarfaraji.sci@gmail.com 100319475328460020988 100319475328460020988 No Razi Clinical Research Development Unit, Razi Hospital, Guilan University of Medical Sciences, Rasht, Iran Hadi Sedigh Ebrahim-Saraie seddigh.hadi@gmail.com 100319475328460020989 100319475328460020989 Yes Department of Microbiology, School of Medicine, Guilan University of Medical Sciences, Rasht, Iran