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Comparison of Injective and Contact Effect of Silver Nanoparticles on The Rate of Hemoglobin Changes in Male Rats
Zahra Amirkhani Dehkordi, Nooshin Naghsh, Heydar Aqbaba,
Volume 1, Issue 1 (10-2012)
Abstract

Background and objective:

The use of silver nanoparticles is one of the functional areas of nanotechnology. These nanoparticles have an antibacterial and antifungal quality. The active ions released form silver nanoparticles can produce free radicals and damage different types of cell. Considering the importance of hemoglobin, this study investigates Comparison of injective and contact effect of silver nanoparticles on the rate of hemoglobin changes in male rats.

Materials and Methods:

In this experimental study were 72 male adult Wistar rats with a mean weight of 225 to 250 grams. The animals were randomly divided into ten groups of eight rats. Nanoparticles were administered to treatment groups using the injective method through intraperitoneal (IP) and the skin contact method with concentrations of 50, 100, 200, and 400 ppm. In order to assimilate the shock resulted by injection and contact, physiologic serum of the same amount was injected to rats of control group or contacted to their skin. Then, blood was drawn from the internal corner of the rats’ eye using capillary tubes. The mean level of hemoglobin in the rats’ blood in the treatment and control group was compared 12 days after the treatment. To compare the means, ANOVA test was used.

Results:

The results showed the dependence of dose and the rate of hemoglobin changes in intraperitoneal injection and the lack of effect of silver nanoparticles on hemoglobin changes in skin contact.

Conclusion:

The effectof the injection of silver nanoparticles on the rate of hemoglobin has been dose-dependent. But the skin contact method has had no effects on the rate of hemoglobin. It seems that a low concentration of nanoparticles and a short period of time cause a lack of effect on the factor.
The experimental model of diet-induced Wistar rat of non-alcoholic fatty liver disease
Jahanbakhsh Asadi , Asra Askari , Zeinab Mohammadi , Babaisan Askari ,
Volume 13, Issue 2 (5-2025)
Abstract
Background: The higher occurrence of NAFLD is associated with insulin resistance, obesity, and diabetes. A diet with high fat or sucrose serves as a NAFLD inducer in rats, which exhibits significant variability and generally results in low levels of liver inflammation and fibrosis. Our study highlights the role of combining animal fats with high sucrose in inducing NAFLD in Wistar rats.
Methods: Twenty male Wistar rats, 8 to 10 weeks old, were randomly divided into two groups: Control (healthy) and NAFLD induction. Weekly weight changes were recorded. After one week of adapted feeding, a 30% vegetable oil and 10% solid sugar diet was added to standard pellets to induce the NAFLD model. After ten weeks, the weight/time of progression of each mouse was measured. Serum and tissue samples were separated and stored for biochemical and histopathological studies. Comparisons were made using independent t-test for two groups with SPSS version 22. The significance level was set at 0.05.
Results: Our results illustrated that weight/time progress increased in the NAFLD-induced group (0.679 g ± 0.02, P < 0.001) more than in the control group (0.559 g ± 0.03). Additionally, FBS (P = 0.001), lipid profile [TG/HDL (P = 0.006), LDL/HDL (P = 0.03), Chol/HDL (P = 0.006), TG (P = 0.005), CHOL (P = 0.001), LDL (P = 0.008), VLDL (P = 0.005), HDL (P = 0.01)], liver function enzymes [ALT (P = 0.001), AST (P = 0.001)[ and IL-6 serum levels were significantly increased in the NAFLD-induced group compared to control rats (P = 0.001). Besides, the obtained results illustrated that the serum level of albumin was significantly decreased in NAFLD-induced rats compared to controls (P = 0.004). Moreover, Oil Red and H&E staining confirmed grade two steatosis induction.
Conclusion: Our study demonstrates significant metabolic abnormalities and steatosis in NAFLD-induced rats, underscoring the detrimental impact of this disease on liver function and overall health. These findings highlight the urgent need for further research into effective interventions for NAFLD.
 

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